Funding tool: Basic research projects
Project-ID: LS12-012
Project start: 01. Oktober 2013
Project end: will follow
Runtime: 36 months / finished
Funding amount: € 215.000,00

Brief summary:

Novel potentiometric sensors for the detection of bioactive nitrogen containing compounds (analytes) based on PVC solid membranes will be developed. The electro-active part of the new membranes consists of an ion-pair complex formed between the protonated analyte (e.g. antibiotic, aminoacids, neurotransmitters, antidiabetic drugs, vitamins) and a borane anion embedded in a plasticizer. The chemical compositions of the active complexes will be elucidated and the analytical parameters of the prepared electrodes, such as slope, concentration range, detection limit, life time, and selectivity will be studied and evaluated with respect to the applicability in commercial instruments. The influence of the nature of the used plasticizer matrix (e.g. polarity) on the performance of ion selectivity of the developed device, especially in terms of the working concentration range (linearity range) and membrane life time will be clarified and based on these results the best set-up will be chosen. Subsequently, the ion selective electrodes will be miniaturized and optimized for an application in the pharmaceutical and clinical daily routine to diagnose analytes in complex matrixes such as saliva, urine, blood and plasma. The final stage of the project will therefore be a prototype of a miniaturized and portable device based on a potentiometric detection of the proposed nitrogen bioactive compounds using the newly developed membranes.

Keywords:
nitrogen bioactive compounds, ion selective electrodes, pharmaceutical and clinical applications