Funding tool: Applied research projects
Project-ID: FTI24-A-005
Project start: 01. April 2026
Project end: 31. März 2029
Runtime: 36 months / not yet started
Funding amount: € 359.422,00

Brief summary:
The aim of this project is to develop an analytical toolkit for evaluating the potency, cytotoxicity, off-target effects, immunogenicity, and tissue specificity of lipid nanoparticles (LNPs). By integrating state-of-the-art molecular biology techniques such as RNA sequencing, qPCR, and FACS with advanced physicochemical methods like Taylor Dispersion Analysis and MALDI imaging, the project establishes a comprehensive and standardized approach. Cell-based assays using cell lines (e.g., HepG2, HUVEC) and primary cells (e.g., hepatocytes, PBMCs) will enable precise analysis of LNP effects. The goal is to standardize reproducible methods for evaluating LNP formulations, particularly for RNA-based therapeutics. The interdisciplinary collaboration between RNAnalytics, FH Tulln, and University for Continued Education Krems combines expertise in LNP production, cell biology, and molecular analysis. By the end of the project, a toolkit will be developed, tested as a prototype in relevant laboratory environments, and made accessible to both academic and industrial users. The outcomes significantly contribute to the development of safe, efficient, and targeted therapeutics, enhance the innovative potential of Lower Austria and are directly integrated into academic teaching to strengthen the training of future professionals.

Keywords:
Lipid-Nanopartikel, RNA-Therapien, Immunogenität, Zellbasierte Assays, Zytotoxizität, RNA-Sequenzierung, Gewebespezifität, Molekulare Marker, TDA, LNP-Charakterisierung